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Journal: Current Research in Food Science
Article Title: Modulating the aroma and taste profile of soybean using novel strains for fermentation
doi: 10.1016/j.crfs.2024.100933
Figure Lengend Snippet: Strains used in fermentation.
Article Snippet: PN , ,
Techniques: Bacteria
Journal: bioRxiv
Article Title: Scaling of mouse somitogenesis by coupling of cell cycle to segmentation clock oscillations
doi: 10.1101/2025.01.10.632257
Figure Lengend Snippet: E10.5 embryonic tails were cultured ex vivo and treated with DMSO or the cell cycle inhibitor Aphidicolin. A-C After 23 hr of incubation, tails were fixed and stained for Brachyury and counterstained with DAPI. Scale bar 100 µm. A Representative images. B Quantification of the Brachyury signal along the AP axis of the PSM. Signal was normalized between 0 and 1. n(DMSO) = 6, n(Aphidicolin) = 11. C Quantification of the length of Brachyury gradient (Line: mean, shading: standard deviation). Intensity normalized between 0 and 1. * < 0.05; *** < 0.001; **** <0.0001. D,E Fluorescence real-time imaging of embryonic tails expressing Achilles-Hes7 was performed. D Representative kymographs of control and Aphidicolin-treated samples. E Quantification of PSM length by determining the size of the oscillating field (Line: mean, shading: standard deviation). Length normalized to the PSM length at experiment start. n(DMSO) = 9, n(Aphidicolin) = 11? F After 23 hr of incubation, tails were fixed and HCR against Hes7 and Uncx4.1 as well as counterstaining with DAPI were performed. Representative images are shown (same as in Fig. S14J). G-J After 23 hr of incubation, tails were fixed and stained with DAPI. G Representative images are shown. H-J Quantification of PSM length from tail tip to last formed somite boundary ( H ), somite length ( I ) and the ratio of somite length to PSM length as a measure of scaling ( J ). K Quantification of cell motility in fluorescence real-time imaging data of embryonic tails expressing Achilles-Hes7 (data corresponds to D,E). L Schematic model of how coupling of cell proliferation to segmentation clock affects somitogenesis.
Article Snippet: For perturbation experiments on whole mount and spread-out tail tips, the following compounds were used: CHIR99021 (Chiron, Sigma-Aldrich, SML1046) at 10 µM, IWP-2 (Sigma-Aldrich, I0536) at 1 µM,
Techniques: Cell Culture, Ex Vivo, Incubation, Staining, Standard Deviation, Fluorescence, Imaging, Expressing, Control